Multiplex Characterization of Individual Extracellular Vesicles
Extracellular vesicles are highly heterogeneous, yet conventional analytical techniques measure millions of vesicles simultaneously, masking biologically important subpopulations
Key advantages of NP-SIMS
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Analyze individual extracellular vesicles instead of population averages
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Reveal molecular heterogeneity and rare EV subpopulations
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Simultaneously detect multiple protein biomarkers on single vesicles
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Differentiate cancer-derived from normal EVs through multiplex molecular profiling
Supporting publication
ACS Nano, (2023), Analytical Chemistry (2021)
Multiplex NP-SIMS analysis of individual extracellular vesicles labeled with lanthanide-tagged antibodies.
Multiplex NP-SIMS analysis of individual extracellular vesicles labeled with lanthanide-tagged antibodies.
Characterization of Smart Drug-Delivery Nanoparticles
Guiding the engineering and scale-up of smart drug delivery systems depends on our ability to characterize individual nanoparticles. Conventional bulk analytical methods mask particle-to-particle differences that ultimately determine therapeutic performance.
Increased DNA loading in gold nanostars compared with spherical gold nanoparticles, demonstrating direct characterization of nanoparticle architecture at the single-particle level.
Increased DNA loading in gold nanostars compared with spherical gold nanoparticles, demonstrating direct characterization of nanoparticle architecture at the single-particle level.
Multiplex NP-SIMS analysis of individual extracellular vesicles labeled with lanthanide-tagged antibodies.
Key advantages of NP-SIMS
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Characterize individual nanoparticles instead of populations averages
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Perform label free molecular characterization
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Resolve cargo loading, surface functionalization and core/shell architectures
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Reveal heterogeneous or incomplete nanoparticles populations
Supporting publication
Analytical Chemistry, (2019)
Multiplex NP-SIMS analysis of individual extracellular vesicles labeled with lanthanide-tagged antibodies.
